Drug test MDMA Rapid test strip,4mm strip detecting Ecstasy in
urine, Quickly, Gold colloidal method
Methylenedioxymethamphetamine (Ecstasy) is a designer drug first
synthesized in 1914 by a German drug company for the treatment of
obesity.1 Those who take the drug frequently report adverse
effects, such as increased muscle tension and sweating. MDMA is not
clearly a stimulant, although it has, in common with amphetamine
drugs, a capacity to increase blood pressure and heart rate. MDMA
does produce some perceptual changes in the form of increased
sensitivity to light, difficulty in focusing, and blurred vision in
some users. Its mechanism of action is thought to be via release of
the neurotransmitter serotonin. MDMA may also release dopamine,
although the general opinion is that this is a secondary effect of
the drug (Nichols and Oberlender, 1990). The most pervasive effect
of MDMA, occurring in virtually all people who have taken a
reasonable dose of the drug, is to produce a clenching of the jaws.
The MDMA One Step Ecstasy Test Strip (Urine) is a rapid urine
screening test that can be performed without the use of an
instrument. The test utilizes a monoclonal antibody to selectively
detect elevated levels of Ecstasy s in urine. The MDMA One Step
Ecstasy Test Strip (Urine) yields a positive result when
Methylenedioxymethamphetamine in urine exceeds 500 ng/mL
The MDMA One Step Ecstasy Test Strip (Urine) is a rapid
chromatographic immunoassay based on the principle of competitive
binding. Drugs which may be present in the urine specimen compete
against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary
action. Methylenedioxy-methamphetamine, if present in the urine
specimen below 500 ng/mL, will not saturate the binding sites of
antibody-coated particles in the test strip. The antibody-coated
particles will then be captured by immobilized
Methylenedioxymethamphetamine conjugate and a visible colored line
will show up in the test line region. The colored line will not
form in the test line region if the Methylenedioxymethamphetamine
level exceeds 500 ng/mL because it will saturate all the binding
sites of anti- Methylenedioxymethamphetamine antibodies.
A drug-positive urine specimen will not generate a colored line in
the test line region, while a drug-negative urine specimen or a
specimen containing a drug in a concentration less than the cut-off
will generate a colored line in the test line region. To serve as a
procedural control, a colored line will always appear at the
control line region, indicating that proper volume of specimen has
been added and membrane wicking has occurred.
DIRECTIONS FOR USE
Bring tests, specimens, and/or controls to room temperature
(15-30°C) before use.
Remove the test from its sealed pouch, and place it on a clean,
level surface. Label the test with patient or control
identification. For best results, the assay should be performed
within one hour.
Using the provided disposable pipette, transfer 3 drops of specimen
(approximately 120 µL) to the specimen well (S) of the device and
start the timer.
Avoid trapping air bubbles in the specimen well (S), and do not add
any solution to the result area.
As the test begins to work, color will migrate across the membrane.
Wait for the colored band(s) to appear. The result should be read
at 5 minutes. Do not interpret the result after 8 minutes
INTERPRETATION OF RESULTS
(Please refer to the illustration above)
NEGATIVE:* A colored line appears in the Control region (C) and
colored lines appears in the Test region (T). This negative result
means that the concentrations in the urine sample are below the
designated cut-off levels for a particular drug tested.
*NOTE: The shade of the colored lines(s) in the Test region (T) may
vary. The result should be considered negative whenever there is
even a faint line.
POSITIVE: A colored line appears in the Control region (C) and NO
line appears in the Test region (T). The positive result means that
the drug concentration in the urine sample is greater than the
designated cut-off for a specific drug.
INVALID: No line appears in the Control region (C). Insufficient
specimen volume or incorrect procedural techniques are the most
likely reasons for Control line failure. Read the directions again
and repeat the test with a new test card. If the result is still
invalid, contact your manufacturer.
A procedural control is included in the test. A line appearing in
the control region
(C) is considered an internal procedural control. It confirms
sufficient specimen volume, adequate membrane wicking and correct
Control standards are not supplied with this kit. However, it is
recommended that positive and negative controls be tested as good
laboratory practice to confirm the test procedure and to verify
proper test performance.
A drug-free urine pool was spiked with Ecstasy at the following
concentrations: 0 ng/mL, 250 ng/mL, 375 ng/mL, 500 ng/mL, 625
ng/mL, 750 ng/mL and 1,000 ng/mL. The result demonstrates > 99%
accuracy at 50% above and 50% below the cut-off concentration.
Results are presented in Table 2 below.
Table 2: Analytical Sensitivity Summary
|MDMA Concentration (ng/mL)||Percent of Cut-off||n||Visual Result|
Conclusion: As indicated in table above: all specimens with MDMA concentration
equal to or lower than 250ng/mL show negative results, all
specimens with MDMA concentration of 500ng/mL are identified as
“+/-”, and all specimens with MDMA concentration equal to or higher
than 750ng/mL showed positive results. Therefore, the cut-off
concentration of the MDMA One Step Ecstasy Test Strip (Urine) is
determined to be 500ng/mL MDMA.
Table 3 lists the compounds that are positively detected in urine
by the MDMA One Step Ecstasy Test Strip (Urine) at 5 minutes and
the concentrations at which they are detected.
Table 3: Analytical Specificity
|(±) 3,4-Methylenedioxymethamphetamine HCl (MDMA)||500|
|(±) 3,4-Methylenedioxyamphetamine HCl (MDA)||3,000|