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Carboxypeptidase B Sequence Grade Carboxypeptidase B for Mass Spectrometry

Carboxypeptidase B Sequence Grade Carboxypeptidase B for Mass Spectrometry

Categories Recombinant Carboxypeptidase B
Brand Name: YaxinBio
Model Number: SRCPB01
Certification: ISO 9001
Place of Origin: China
MOQ: 100ug
Payment Terms: T/T
Packaging Details: ice packaging
Price: 198$/100ug
Supply Ability: 1000g per week
Product: Sequencing Grade Carboxypeptidase B
Manufacturer: YaxinBio
Physical form: Lyophilized powder
Specific activity: NLT 200 units/mg pro
Contaminant activity: No trypsin, chymotrypsin, carboxypeptidase A, or other proteases contaminant.
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    Carboxypeptidase B Sequence Grade Carboxypeptidase B for Mass Spectrometry

    Carboxypeptidase B Sequence Grade Carboxypeptidase B for Mass Spectrometry


    Description


    Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine

    from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum

    is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme

    is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed

    in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme

    (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are

    present in the preparation.


    Main Features


    Source

    E. Coli

    Purity by HPLC

    ≥ 95%

    Format

    Lyophilized

    Contaminant activity

    No chymotrypsin, carboxypeptidase A, or other proteases contaminant.

    Formulation:The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5.

    Solubility:It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile

    18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

    Purity: Greater than 95% as determined by SDS-PAGE.

    Unit Definition: One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C,

    pH-7.65.


    Usage


    ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may

    not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


    Stability


    Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below 2-8°C. For long term storage it is recommended to be stored below -20℃.Please prevent freeze-thaw cycles.

    Quality Carboxypeptidase B Sequence Grade Carboxypeptidase B for Mass Spectrometry for sale
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