Carboxypeptidase B Sequence Grade Carboxypeptidase B for Mass
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids
lysine, arginine and histidine
from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum
is 8.0, and pI is 6.0. Carboxypeptidase B is competitively
inhibited by arginine and lysine. The enzyme
is also inhibited by metal chelating agents, e.g., EDTA.
Recombinant Carboxypeptidase B is expressed
in E.Coli and purified by high pressure liquid chromatography.
There is no trace of other enzyme
(such as carboxypeptidase A and chymotrypsin) activity. No protease
inhibitors such as PMSF are
present in the preparation.
Purity by HPLC
No chymotrypsin, carboxypeptidase A, or other proteases
Formulation:The protein was lyophilized with 100mM NaCl and 20mM
Solubility:It is recommended to reconstitute the lyophilized Rat
Carboxypeptidase-B in sterile
18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to
other aqueous solutions.
Purity: Greater than 95% as determined by SDS-PAGE.
Unit Definition: One Unit hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25°C,
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY.
The product may
not be used as drugs, agricultural or pesticidal products, food
additives or household chemicals.
Recombinant Rat Carboxypeptidase-B although stable at room temp for
1 week, should be stored desiccated below 2-8°C. For long term
storage it is recommended to be stored below -20℃.Please prevent