HCV Quantitative RT-PCR Kit

INTENDED USE

The kit is used for the qualitative or quantitative detection of HCV RNA in human serum or plasma by Real time RT-PCR method. It can be used to determine the infection of HCV virus, evaluate antiviral treatment and monitor the therapeutic effect by monitoring HCV RNA baseline levels and changes in patient blood. The test results of this kit should be combined with other relevant medical examination results for comprehensive analysis, and should not be used as the diagnosis basis alone.

The kit is for research use only, and intended to be used by trained professionals in a laboratory environment.

SUMMARY

The hepatitis C virus (HCV) is a small, enveloped, single-stranded, positive-sense RNA virus. It is a member of the genus Hepatitis C virus in the family Flaviviridae. HCV is spread primarily by direct contact with human blood. It is a major cause of acute hepatitis and chronic liver disease, including cirrhosis and liver cancer. Detecting HCV RNA in blood will be helpful in determining the infection of HCV virus and monitoring disease progression and response to antiviral therapy.

Technical Parameters:

PRINCIPLE

The conserved region of Hepatitis C virus (HCV) is used to generate probes and primers in this real-time RT-PCR assay. HCV RNA are reverse-transcribed and amplified accurately by the specific primers. At the same time, the highly specific TaqMan probe can be combined with the target nucleic acid fragments, and will be hydrolyzed to produce the fluorescence signals under the action of Taq enzyme exonuclease activity, in which the real-time amplification curve will be obtained according to the relationship between the fluorescence signal and the number of amplification cycles, besides, the false negative and the PCR inhibition can be monitored by Internal Control.

The specific RNA (FAM channel) of HCV be qualitatively detected under the quality control by internal control (ROX channel).

Customization:

Biovantion PCR Reagent is a DNA amplification reagent that is perfect for real-time PCR experiments. The Model Number 5 reagent is made in China and has a concentration of 10X. Primer is not included in this product, and the reaction conditions are standard. The catalog number for this product is K1081. Template DNA is also not included.

Support and Services:

Our PCR Reagent product technical support team is available to answer any questions or concerns you may have regarding the product. We offer troubleshooting assistance for any issues you may experience during the PCR process, as well as guidance on optimal usage of the reagent. Additionally, we provide comprehensive training and education on the product to ensure that our customers are fully informed and equipped to use the PCR Reagent effectively. Our services also include product maintenance and repair, as well as periodic product updates to ensure the highest quality and performance. Please do not hesitate to reach out to us for any technical support or service needs related to the PCR Reagent product.

Packing and Shipping:

Product Packaging:

• 10 ml PCR Reagent in a sterile tube
• Instruction manual

Shipping:

• Shipped in a temperature-controlled packaging to maintain product stability
• Shipped via express delivery to ensure timely delivery
• Shipping cost may vary depending on the destination and shipping method chose