Kit to Evaluate Sperm DNA Integrity

Sperm DNA Fragmentation Sperm Chromatin Structure Assay Kit AOT

Flow Cell Methodology

Packing specification:

• 20 tests/kit

Intended use:

• The kit is designed to evaluate the DNA integrity and maturity of human sperm by staining sperm chromatin.

Test principle:

After denatured by acid, fragmented sperm chromatin would turn to single strand. And the normal sperm chromatin retain double strand. Acridine Orange(AO) associated with single-stranded DNA emits red or yellow fluorescence at 488nm luminescence. While AO intercalated in double stranded DNA emits green fluorescene. If the red fluorescence is increasing, it shows a high rate of DNA fragmentation in sperms.

Main compositions:

• Reagent A 1 vial 2.5 ml
• Reagent B 1 vial 4.5 ml
• Reagent C ( lyophilized powder ) 2 vials 13 ml 6.5ml/vial
• Lyophilized powder dissolved solution 1 vial 4 ml

Storage conditions and duration:

• Storage conditions: The kit is kept at 2 ~ 8 ℃ in dark place.
• Period of validity: The kit is kept at 2 ~ 8 ℃ for one year. After dissolved, the reagent C would be kept for two weeks in dark at 2 ~ 8 ℃. Once separately store the dissolved reagent C at -20 ℃ in dark, the use life of reagent C could be more than one year.

Applicable instrument:

• Flow cytometer (488nm luminescence)

Specimen requirements:

• The sample should be collected after a minimum of 48 hours but not longer than seven days of sexual abstinency by masturbation or sexual intercourse using special condoms. The semen sample must be complete in collection.
• Fresh semen samples should be liquefied completely prior to testing. If the samples have delayed liquefaction or high viscosity, a liquefier (sold separately) should be conducted.

BRED Life Science Technology Inc. specializes in developing, manufacturing and marketing lab products in the field of reproductive medicine, offering automatic assay platform and related products for infertility diagnosis and ART treatment. Based on the test methods and standard recommended by the World Health Organization, BRED develops serial products for infertility diagnosis and ART treatment with innovative technology. And these help promote the establishment and popularization of standardization for reproductive medicine lab. BRED own a completest range of relative products in the field of reproductive medicine worldwide.The products are of independent intellectual property right within BRED.

Our mission of development is to be devoted to providing the best solutions and products for the clinical laboratory in reproductive medicine with the aim to promote the development of human reproductive health.

Our operating philosophy is putting people first, doing business on the basis of honesty, forging ahead and working with diligence.

Welcome to visit our official website: www.spermfunc.com