Iron Stain
Intended Use:
This kit is for staining bone marrow cell and bloodcell smear.
Principle:
Iron is stored in bone marrow as hemosiderin which makes nucleated
erythrocytes to synthesize to hemoglobin. Iron stored outside of
red blood cells is called extracellular iron. Iron granules are
found in somepolychromatophilic normoblast, orthochromatic
normoblast and mature red blood cells. Those cells are respectively
called sideroblasts and siderocytes which belong tointracellular
iron. This iron stain is based on the Prussian blue reaction. Acid
potassium ferrocyanide reacts with intracellular iron and
extracellular iron to form an insoluble blue ferric ferrocyanide
precipitate in iron depots of cells.
Specifications:
| Contents | 5Tests/Kit | 20Tests/Kit | 100Tests/Kit | Components |
| Potassium Ferrocyanide (solution A) | 1vial*5ml | 1vial*20ml | 1vial*100ml | Potassium ferrocyanide |
| Hydrochloric Acid (solution B) | 1vial*5ml | 1vial*20ml | 1vial*100ml | Hydrochloric Acid |
| Nuclear Fast Red (solution C) | 1vial*5ml | 1vial*20ml | 1vial*100ml | Nuclear Fast Red |
Methods:
Working solution Preparation (for 1 test only): Add1ml of solution A and solution B into a tube. Mix thoroughly.
