BeaverBeads NHS With Good Bio Compatibility And Low Non Specific Adsorption

BeaverBeads NHS with Good Bio-compatibility and Low Non-specific Adsorption​

1. Description

BeaverBeads™ NHS are superparamagnetic beads with activated groups of N-Hydroxysuccinimide on the surface, which can react with primary amines on desired ligands and bind them covalently with amide linkage to the beads. The ligand can be various molecules including antigen, antibody and other proteins. Compared with the traditional carboxyl, amino magnetic beads, the NHS group of magnetic beads don't need to be activated by EDC/NHS or Glutaraldehyde, simply dissolve the amino-containing bioligand in the coupling buffer at room temperature, then mix with proteins and the NHS magnetic beads for 1-2 hours, the bioligand can be coupled to the magnetic beads. The advantages include easy operation, mild coupling conditions and efficient coupling. The magnetic bead
coupling process must be carried out in a buffer solvent free of any amino. This method can be operated manually or automatically.

2. Features

• Convenient. Direct covalent binding with bioligand, no pre-activation needed
• Efficient. The efficiency of binding with bioligand is up to 90% which is higher than carboxyl magnetic
  beads. Binding capacity is much higher than others'
• Rapid. Complete the bioligand coupling in 1-2 h
• Mild. Binding temperature is RT or 4 ℃ and pH is between 5 and 9
• Stable. Form a stable amide bond in order to prevent ligand shedding
• Good bio-compatibility and low non-specific adsorption

3. Application Case

Use enzyme chemiluminescence method for antibody quantitative detection, the results show that BeaverBeads™ Mag NHS achieve 20% higher sensitivity than the competitive products, showing excellent chemiluminescence performance.

• In-vitro diagnostic
• Immunoassay
• Cell isolation
• Immuno-precipitation
• Protein (antibody) purification

4. Product List

BEAVER was founded in the end of 2011, located at Suzhou Industrial Park. BEAVER combines biotech with nanotech as a “twin-turbine”strategy in the product design. BEAVER team focuses on magnetic nanoparticle technology and product development, provides customers with professional sample processing solutions, and is committed to promoting the development of life science industry. BEAVER provides high-quality products to customers from Europe, North America, Mid East, etc. BEAVER’s products are used by over 1000 institutions, 300 enterprises and cited by over 200 papers. BEAVER has been cooperating with domestic and overseas experts for nanomaterial industry international standards ISO 19807-2, projected to be published in 2021. In addition, BEAVER has established a quality management system and passed ISO 9001 and ISO 13485. BEAVER products also have CE and NMPA Class I certificates. BEAVER carries on the quality control to each step of the product life cycle, in order to ensure that the product quality conforms to the regulation and the customers’ needs.